First Floyd Rose Equipped Guitar

I am having a few problems with tuning, if somebody can give me some advice it would be greatly appreciated. I am tuning up starting Low E then A the back to E, A then D back to E, A, D then G etc until all strings have been tuned. By the time I have finished the High E and try to check the tuning on the Low E this has flattened to around an F. So when I have locked down the strings and tune using the Micro Tuners I don't have enough adjustment available to bring this back into tune even if I leave them all the way out. Also is there any reason why when I adjust the micro tuners this puts the other strings out. If I am just being a clueless Muppet when it comes to a Floyd Rose can you please let me know. Any advice/step by step instruction would be very welcome. It is incredibly annoying having bought a new Guitar about 2 weeks ago and not being able to play it. Thanks for you help. Hope to speak to you soon.

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